Table 1.

Summary of continuous cultures used as a tool toward the rational based bioprocess development with P. pastoris cell factory.

Protein expressed	Strain	Promoter	Substrate	Continuous strategy	Production kinetics	Optimization approach	References
Fab fragment (anti-HIV antibody 2F5)	X-33	GAP	Glucose	Chemostat	Growth-coupled μ-saturated	Optimized feeding from continuous data	Maurer et al., 2006
Fab fragment (anti-HIV antibody 3H6)	X-33	GAP	Glucose	Chemostat	Growth-coupled μ-saturated	Optimized feeding from continuous data	Buchetics et al., 2011
Fab fragment (anti-HIV antibody 2F5)	X-33	GAP	Glucose	Chemostat	Growth-coupled	Optimal D at continuous mode	Garcia-Ortega et al., 2016
hGM-CSF (human)	GS115 Mut+	GAP	Glucose	Chemostat	Linear	Fed-batch designed from continuous data	Khasa et al., 2007
phytase	X-33	GAP	Glucose	Chemostat	Linear	Fed-batch designed from continuous data	Tang et al., 2010
srAFP	GS115 MutS	AOX	Glycerol/methanol	Chemostat	Growth-coupled	Fed-batch designed from continuous data	D'Anjou and Daugulis, 2001
°scFv	GS115 Mut+	AOX	Methanol	DO-stat S-stat	Bell shaped μ-saturated	Trade-off between fed-batch and continuous	Yamawaki et al., 2007
hCTRB	GS115 Mut+	AOX	Methanol	Nutriostat	Linear	Optimal D at continuous mode	Curvers et al., 2002
rHV2	GS115 Mut+	AOX	Methanol	Chemostat	Bell shaped	Fed-batch designed from continuous data	Zhou and Zhang, 2002
Porcine trypsinogen	X-33	AOX	Glucose/methanol	Chemostat	Bell shaped	Optimal D at continuous mode	Paulová et al., 2012
Avidin	GS115 Mut+	AOX	Methanol/glycerol/ sorbitol	Chemostat	Linear	Fed-batch designed from continuous data	Jungo et al., 2006, 2007a,c
CalB	X-33	PGK	Glycerol	Chemostat	Linear	Trade-off between fed-batch and continuous	de Macedo Robert et al., 2019
Serum albumin (human)	X-33	THI11	Glucose	Chemostat	Growth-coupled	Optimized feeding from continuous data	Landes et al., 2016