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. 2020 Jun 25;14:613. doi: 10.3389/fnins.2020.00613

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Copyright © 2020 Liu, Qiao, Wang, Wu, Su, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-224-5p Regulated the Expression of NR4A1 in Hypoxic Neurons. (A) The intersection of the predicted target gene results of miR-224-5p in miRDB, TargetScan, miRanda, and miRmap. The different color represents the distinctive method of prediction. There were three target genes of miR-224-5p, including NR4A1, RNF38, and IL1RN. (B) QRT-PCR analyses revealed that the mRNA expression of NR4A1 was decreased in hypoxic neurons (^∗∗P < 0.01). (C) ELISA analyses revealed that the protein expression of NR4A1 was decreased in hypoxic neurons (^∗∗P < 0.01). (D) Alignment of the seed regions of miR-224-5p with 3′-UTR of NR4A1. The red color indicated the pairing bases. (E) The relative luciferase activity was significantly decreased after treatment with miR-224-5p, whereas the inhibitory effect of miR-224-5p was abolished in the plasmid containing the mutant 3′-UTRs of NR4A1.