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. 2020 Jun 25;14:613. doi: 10.3389/fnins.2020.00613

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Copyright © 2020 Liu, Qiao, Wang, Wu, Su, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of MiR-224-5p Ameliorated Neurodegeneration Through NR4A1. (A) Tuj1⁺ neurons (green) were observed in the control group, OGD group, anti-miR-224-5p group, anti-miR-224-5p + si-NC group, anti-miR-224-5p + si-NR4A1 group, and anti-NC + si-NR4A1 group. Nuclei were visualized by DAPI (blue). (B) The number of Tuj1⁺ neurons was quantified. The number of Tuj1⁺ cells was decreased in the anti-miR-224-5p + si-NR4A1 group compared with the anti-miR-224-5p + si-NC group (^∗∗P < 0.01). (C) The neurite length was also quantified. The axonal length of neurons was decreased in the anti-miR-224-5p + si-NR4A1 group compared with the anti-miR-224-5p + si-NC group (^∗∗P < 0.01).