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. 2020 Jul 2;56(1):1901458. doi: 10.1183/13993003.01458-2019

FIGURE 3.

FIGURE 3

a and b) Example image, trace and graph of the GSK1016790A-induced increase in calcium in human airway smooth muscle cells (HASMCs). Example ptychography reconstituted images (top panel) and traces (bottom panel) from (c) GSK1016790A (100 nM) treated and (d) acetylcholine (Ach) (10 µM) treated HASMCs (n=2 donors). e) Ptychography data indicating no contractile effect to GSK1016790a in human HASMCs alone. ACh was used as a positive control (n=2 donors). f) Reconstituted ptychographic images; top two panels show HASMCs alone. Bottom two panels show HASM co-cultured with mast cells, where the addition of GSK1016790A (100 nM) led to an increase in optical density. g) In the presence of mast cells, GSK1016790A (100 nM) caused contraction of HASMCs (n=2 donors for HASMCs, n=3 donors for human lung mast cells (HLMCs)). Data are presented as mean±sem (n=8–56 readings;). Scale bar indicates 100 µm. *: p<0.05 using a Kruskall Wallis test with Dunn's post comparison test comparing responses to vehicle (b) or a t-test comparing responses to vehicle control (g).