FIGURE 4.

a) TRPV4 mRNA is expressed on human airway smooth muscle cells (HASMCs), but not on human lung mast cells (HLMCs). b) GSK1016790A (100 nM) induced ATP release from HASMCs which was inhibited by GSK2193874 (10 µM). c) ATP contraction of isolated human tracheal strips was inhibited by montelukast and zileuton (10 µM). Data are shown as mean±sem. HASMCs were isolated from 4 patients, tracheal strips from 3 donors' lungs. *: statistical significance (p<0.05); t-test comparing responses to relevant control (b) or using a one-way ANOVA with Dunnett's multiple comparison test comparing responses to vehicle (c).