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. 2020 Jun 25;11:1200. doi: 10.3389/fimmu.2020.01200

Figure 6.

Figure 6

mTOR is a cell-intrinsic regulator of IFN-γ synthesis in NK cells exposed to S. aureus. (A) Cultures of PBMC from healthy donors (n = 3) were set up in serum from control subjects (c) or from trauma patients (t) and were stimulated with S. aureus. Unstimulated cells served as control (none). The mean fluorescence intensity (MFI) of phosphorylated mTOR was determined in gated CD3−CD56bright NK cells after 18 h. Horizontal lines indicate the median/interquartile range. (B–D) Purified NK cells from healthy donors (n = 2–4) were exposed to conditioned medium of PBMC from healthy donors that had been obtained after stimulation with S. aureus (2–3 different batches). Rapamycin (rapa) was added or not (–). The percentage of CD56bright NK cells positive for IFN-γ (B), IL-12Rβ2 (C), and CD117 (D) was quantified. Statistical differences were tested using the Wilcoxon signed rank test. *p < 0.05.