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. 2019 Mar 7;74(7):1031–1042. doi: 10.1093/gerona/glz061

Figure 3.

Figure 3.

Aged animals (22–24 mo old) received intraperitoneal injections of recombinant mouse α-Klotho (1.05 μg/200 μL injection; R&D Systems, 1819-KL, aa 35-982) or isotonic saline for 6 consecutive days immediately after injury. (A) Force-frequency curves obtained via in situ contractile testing of anterior compartment strength (n = 5–6/group; two-way repeated measures analysis of variance, *p < .05; one animal removed from the saline group for analysis due to electrode misplacement). (B) Hang impulse (calculated as hanging time (s) × mouse bodyweight [g]) at 14 d post-injury (n = 5–6/group; *p < .05, one-tailed Student’s t-test).