Figure 4.

Zinc Distribution and Quantification Over Oocyte Maturation and Egg Activation in w; znt35C¹Drosophila

(A–D) The total zinc content (A and C) and zinc concentration (B and D) in each egg chamber (A and B) and oocyte (C and D) of w; znt35C¹ compared with ORP2 wild-type. WT: ORP2 wild-type. KO: w; znt35C¹ null mutant. Stage 1–8 oocytes: WT n = 11, KO n = 15; stage 9–13 oocytes: WT n = 15, KO n = 20; stage 14 oocytes: WT n = 14, KO n = 19; activated eggs: WT n = 19, KO n = 12. a, b, c, d, and e: significance groups (significant difference of mean with p < 0.05 between groups with different labels). Whiskers on the boxplots show the top and bottom quartiles. (E and F) Representative XFM images showing zinc distribution in (E) a mature oocyte at stage 14 and (F) an activated but unfertilized egg from w; znt35C¹ mutant females. Scale bars, 100 μm. (E′ and F′) Enlarged view of (E and F). Dashed squares indicate enlarged regions. Scale bars, 10 μm.