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. 2020 Jun 16;23(7):101274. doi: 10.1016/j.isci.2020.101274

Figure 3.

Figure 3

Therapeutic Effect of Nanoreactors for HeLa Cells

(A) Confocal fluorescent images of HeLa cells after incubation with ZIF-8@GOx-AgNPs@MBN at different times (n = 3). All scale bars, 80 μm.

(B) Flow apoptosis assay of HeLa cells after different treatment times (n = 3).

(C) The confocal fluorescent images of live and dead HeLa cells that underwent different treatments, including control (PBS), ZIF-8, ZIF-8@GOx, AgNPs@MBN, and ZIF-8@GOx-AgNPs@MBN. Cells were stained by Calcein-AM/propidium iodide, and the green and red colors stand for the alive and dead cells, respectively (n = 3). All scale bars, 80 μm.

(D) SERS spectra of HeLa cells incubated with ZIF-8@GOx-AgNPs@MBN for different times.

(E and F) Relative cell viabilities of HeLa cells incubated with different concentrations of Ag+, Zn2+ (E), and Ag+/Zn2+ with or without glucose for 24 h (F), determined by the MTT assay.