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. 2020 Jun 30;6(6):e04347. doi: 10.1016/j.heliyon.2020.e04347

Figure 2.

Figure 2

Characterization of BMSCs and SDF-1α/CS/GP/HEC hydrogels provide sustained release of bioactive SDF-1α. (A) Flow cytometric analysis of BMSCs surface markers for CD29, CD44, CD90, CD105, CD34 and CD45. (B) BMSCs cultured in osteogenic induced media, mineralized nodules detected by Alizarin Red staining. (C) Cultured BMSCs formed Oil Red O-positive lipid droplets of adipogenic induction. Black arrow, lipid droplets. (D) SDF-1α release from SDF-1α/CS/GP/HEC hydrogels is sustained over 30 days in vitro. (E) Migration of BMSCs in response to the release media collected at 3, 6 and 9 days in a transwell assay. (F) The migrated cell number of BMSCs in release media. Data are presented as mean ± SD. n = 3. ∗∗p < 0.01, ∗∗∗p < 0.001 represent significant differences.