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. 2020 Jun 30;6(6):e04347. doi: 10.1016/j.heliyon.2020.e04347

Figure 4.

Figure 4

Histological analysis of newly formed bone and polarization states of macrophages in cervical lymph nodes. (A) Representative H&E staining images displaying the newly formed tissues in vivo at 8 weeks post-surgery. NB, newly formed bone. Root, the mesial root of first molar. The black arrows pointed to the primary bone tissues or bone trabecula-like tissues. The white arrows pointed to fibrous connective tissues. (B, C) Masson staining for the new bone and fibers and quantitative analysis. (D, E) Western blot for the related protein expression and its quantitation. (F, G) 8 weeks after transplantation, gene expression in cervical lymph nodes of IL-1β, TNF-α and iNOS (M1 phenotype-related) (F) and CD206, IL-10 and Arg1 (M2 phenotype-related) (G). Data are presented as mean ± SD. n = 5 for staining; n = 3 for qRT-PCR and western blot. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 represent significant differences.