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Physiochemical substrate properties (a) Principal component (PC) analysis of defined medium (DM) metabolites which were incubated with different substrates. The metabolite relative abundance in percent was normalized by defined medium incubation without substrate, and the recovery rate is given in brackets (means ± SEM, n = 3). (b) Diffusion of colorimetric dye through various substrates as determined by the advancement of the dye front over time
