. 2020 Jun 22;2020:4234632. doi: 10.1155/2020/4234632

Table 2.

Primer sequences for RT-PCR and RT-qPCR.

Gene		Primer sequences (5′-3′)	Product size (bp)	Annealing T (°C)
FOXA1	Forward	CGCTTCGCACAGGGCTGGAT	143	62
FOXA1	Reverse	TGCTGACCGGGACGGAGG AG	143	62
E-cadherin	Forward	CGGGAATGCAGTTGAGGATC	199	60
E-cadherin	Reverse	AGGTGGTGTAAGCGATGGC	199	60
N-cadherin	Forward	ATTGGACCATCACTCGGCTTA	158	58
N-cadherin	Reverse	CACACTGGCAAACCTTCACG	158	58
LMP1	Forward	AGCCCTCCTTGTCCTCTATTCCTT	253	60
LMP1	Reverse	ACCAAGTCGCCAGAGAATCTCC	253	60
TWIST	Forward	CAAGCTGCAGCTATGTGGC	168	57
TWIST	Reverse	TGTCCATTTTCTCCTTCTCTGG	168	57
Vimentin	Forward	CCCTCACCTGTGAAGTGGAT	271	60
Vimentin	Reverse	TCCAGCAGCTTCCTGTAGGT	271	60
Snail	Forward	GATGCCGCGCTCCTTCC	265	61
Snail	Reverse	GGGGACTCACTCGCCCC	265	61
GAPDH	Forward	GCTCTCTGCTCCTCCTGTTC	122	60
GAPDH	Reverse	CGCCCAATACGACCAAATCC	122	60