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. 2020 Aug;98(2):72–87. doi: 10.1124/mol.119.118448

Fig. 6.

Fig. 6.

CXCR4 homodimers undergo conformational changes in response to CXCL12. (A) The rearrangement between CXCR4 protomers was investigated in HEK293 cells cotransfected with CXCR4-CFP and CXCR4-YFP, with the fluorophores fused to the C termini. (B) Representative traces of the FRET response from a single HEK293 cell expressing CXCR4-YFP and CXCR4-CFP and stimulated with 30 μM CXCL12 (black line). Upper panel shows corrected YFP (yellow) and CFP (cyan) emissions. Lower panel shows corrected and normalized FRET ratio. (C and D) On-kinetics of the rearrangement between CXCR4 protomers in response to CXCL12 (C) and off-kinetics upon wash-out of the ligand with buffer (D). τ alues from individual experiments are represented in a scatter plot with median and IQR. N = 28 and 10 cells, respectively, measured on 4 independent experimental days. a.u., arbitrary units.