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. 2020 May 25;11(16):4724–4735. doi: 10.7150/jca.33232

Figure 1.

Figure 1

Exosomes derived from hypoxic CRC cells increase the proliferation, migration, and invasion of normoxic CRC cells. (A) Electron micrograph of exosomes isolated from HT29 exosome-free medium under normal or hypoxia revealing the typical morphology and size. (B) NTA of HT29-Hy-exo or HT29-Nor-exo isolated by ultracentrifugation. (C) Western blot analysis showing the presence of CD81, TSG101, CD9, and CD63 in exosomes derived from normoxic or hypoxic HT29 and DLD1 cells. (D) Representative immunofluorescence image shows the internalization of PKH67-labeled HT29-derived exosomes (green) under hypoxia by normoxic HT29 cells. (E) (F), and (G) Cell proliferation, migration and invasion capacity of CRC cells (DLD1 and HT29) treated with normoxic or hypoxic exosomes was determined by the colony formation, wound healing assay and transwell invasion assay, respectively. (H) (I), and (J) Cell proliferation, migration and invasion capacity of CRC cells (DLD1 and HT29) treated with exosomes isolated from hypoxic medium with or without GW4869 was determined by the colony formation, wound healing assay and transwell invasion assay, respectively. Representative photographs of migratory or invaded cells (magnification, ×200) are shown; Error bars, SD. ***P < 0.001.