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. Author manuscript; available in PMC: 2021 May 7.
Published in final edited form as: Lab Chip. 2020 Apr 16;20(9):1586–1600. doi: 10.1039/d0lc00099j

figure 6.

figure 6.

Lymphatic vessel co-culture with metastatic breast cancer cells (MDA-MB-231) in LD and HD matrices. a) schematic of the experimental conditions and timeline. b) top-view and cross-section view of immunofluorescent images of lymphatic vessels co-cultured with metastatic breast cancer cells in LD (left) and HD (right) matrices (f-actin in purple, cd31 in red, MDA-MB-231-GFP in green and nuclei in blue. dashed outlines indicate endothelial cell detachment in the vessel wall. c) legend and cross-section view schematic of conditions. d) co-cultures IL-6 protein secretion levels in LD and HD matrices. e) quantification of solute permeation for lymphatic lumens in LD and HD matrices and for lymphatic lumens in LD and HD matrices treated with anti-Igg1 (control) and anti-il-6r (blocking treatment). bars represent average ± SD, n at least 3 individual vessels. (n =4, pooled samples over 2 days from at least 6 lumens). scale bar = 140 μm. *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.