Figure 3.

KC depletion completely protects against concanavalin A (Con A)-induced acute liver injury associated with reduced inflammatory cytokines in serum. (A) Scheme of experimental procedures for the mouse genotypes, and the timeline of ILY and Con A treatments and further analysis. ihCD59⁺ and LysM-Cre⁺ihCD59⁺ mice were treated with 1 ILY (middle, 150 ng/g, i.p.) or 3 ILY injections (right, 100 ng/g, i.p. at 2 h intervals), followed by the injection of Con A (12 mg/kg i.v.) 24 h later. Serum were collected at 4 and 8 h after Con A injection by tail bleeding. (B) H&E stained liver sections and serum ALT and AST of ihCD59⁺ (n = 3) and LysM-Cre⁺ihCD59⁺ (n = 3) at 24 h after the Con A injection. Left panel is another experimental control group treated with 1 ILY injection (200 ng/g, i.p., n = 2) only. (C) Serum level of cytokines at 4, 8 and 24 h after Con A treatment in ihCD59⁺ and LysM-Cre⁺ihCD59⁺ mice were analyzed by Legendplex flow assay (n = 6 for 4 and 8 h and n = 2 for 24 h). Data are shown as mean ± SEM. ** P<0.01; *** P<0.0005; **** P<0.0001 (two-way ANOVA) in comparison with ihCD59⁺ group. # P<0.05; ## P<0.01 as determined by unpaired two-tailed Student's t-test.