Table 3.

List of practical primer design tools

Aim	Name	Software or Website
Secondary structure prediction	Mfold	http://mfold.rna.albany.edu/?qZmfold
	RNAfold	http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi
	RNAStructure	http://e.informer.com/rna.urmc.rochester.edu/RNAstructure.html
	SFold	http://sfold.wadsworth.org/cgi-bin/index.pl
Primer design	Primer Premier	Software
	Primer Express	Software
	DNAMAN	Software
	Oligo 7	Software
	Prime3 Plus	http://primer3plus.com/cgi-bin/dev/primer3plus.cgi
	QuantPrime	https://www.quantprime.de/
	Primer Blast	https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi
	Primer bank	https://pga.mgh.harvard.edu/primerbank/
	JCVI Primer Designer	https://sourceforge.net/projects/primerdesigner/
Test the primer specificity	NCBI Primer-BLAST	https://www.ncbi.nlm.nih.gov/tools/primer-blast/
	Primer Blast	https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi