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. 2020 Jun 12;10(16):7465–7479. doi: 10.7150/thno.45003

Figure 2.

Figure 2

Generation of podocyte-specific Sirt6 knockout mice. (A) Schematic diagram of the construction of podocyte-specific Sirt6 knockout (Sirt6flox/flox/Nphs2.Cre+) mice. Exons 3-6 of Sirt6 were deleted in podocytes by the Cre-Loxp system. (B) Representative PCR image of tail genotyping of each group. (C) Representative Western blots of Sirt6 and quantification of Sirt6 protein levels in the glomeruli of each group. *P <0.05 versus Control, n=6. (D) Representative immunohistochemistry staining of glomerular Sirt6 in each group (arrows point to the regions of nuclear Sirt6 expression). Scale bars: 20 µm. (E and F) Podocyte-specific loss of Sirt6 was confirmed by immunofluorescent staining for Sirt6 in podocytes. WT1 and Synaptopodin were used as podocyte markers. Control: Sirt6flox/flox/Nphs2.Cre- group; Sirt6.Podo-cKO: Sirt6 podocyte conditional knockout: Sirt6flox/flox/Nphs2.Cre+ group; WT: wild-type; WT1: Wilms' tumor-1. Scale bars: 20 µm.