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. 2020 Jun 12;10(16):7465–7479. doi: 10.7150/thno.45003

Figure 4.

Figure 4

Podocyte-specific deletion of Sirt6 aggravates Ang II-induced cholesterol accumulation in podocytes in vivo. (A) Representative microscopy images and quantification of WT1 and Adrp double staining of kidney sections for each group (original magnification×600). *P < 0.05, n=30. Scale bars: 20 µm. (B) Representative microscopy images and quantification of filipin and WT1 double staining of kidney sections in each group (original magnification ×600). *P < 0.05, n=30. Scale bars: 20 µm. (C) Representative Western blots and quantification of ABCA1, HMGCR and ABCG1 protein levels in each group. *P < 0.05, n=3. ABCA1: ATP-binding cassette transporter A1; ABCG1: ATP-binding cassette transporter G1; Adrp: adipocyte differentiation-related protein; HMGCR: 3-hydroxy-3-methylglutaryl coenzyme A reductase; LDLR: low-density lipoprotein receptor; Control: Sirt6flox/flox/Nphs2.Cre- group; Sirt6.Podo-cKO: Sirt6 podocyte conditional knockout: Sirt6flox/flox/Nphs2.Cre+group; WT1: Wilms' tumor-1.