Skip to main content
. 2020 Jun 12;10(16):7465–7479. doi: 10.7150/thno.45003

Figure 6.

Figure 6

Deletion of Sirt6 affects the protective effect of CD on Ang II-induced podocyte injury in vitro. Podocytes were transfected with scrambled siRNA, or Sirt6 siRNA before pretreatment with 5 mM CD for 1 h and then stimulated with 10-7 M Ang II for 24 h. (A) Representative microscopy images and quantification of Adrp staining in each group (original magnification×600). *P < 0.05, n=20. Scale bars: 10 µm. (B) Representative microscopy images and quantification of filipin staining in each group (original magnification×600). *P < 0.05, n=20. Scale bars: 10 µm. (C) Quantitative analysis of cholesterol content in each group. *P < 0.05, n=9. (D) Quantitative analysis of cholesterol efflux rate in each group. *P < 0.05, n=6. (E) Flow cytometry analysis of the apoptotic rate of podocytes in each group. *P < 0.05, n=4. (F) Representative Western blots and quantification of ABCA1, HMGCR and ABCG1 protein levels in each group. *P < 0.05, n=3. ABCA1: ATP-binding cassette transporter A1; ABCG1: ATP-binding cassette transporter G1; Adrp: adipocyte differentiation-related protein; CD: cyclodextrin; HMGCR: 3-hydroxy-3-methylglutaryl coenzyme A reductase; LDLR: low-density lipoprotein receptor.