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. 2020 Jun 12;10(16):7465–7479. doi: 10.7150/thno.45003

Figure 7.

Figure 7

Sirt6 overexpression interfered Ang II-induced cholesterol accumulation and injury in podocytes in vitro. The podocytes were transfected with no plasmid, pcDNA3.1, or pcDNA3.1-Sirt6 and then stimulated with 10-7 M Ang II for 24 h. (A) Representative Western blots of Sirt6/ABCG1 protein levels in each group, *P < 0.05, n=3. (B) Representative microscopy images and quantification of Adrp and filipin staining in each group (original magnification×600). *P < 0.05, n=20. Scale bars: 10 µm. (C) Quantitative analysis of cholesterol content in each group. *P < 0.05, n=8. (D) Quantitative analysis of cholesterol efflux rate in each group. *P < 0.05, n=12. (E) Flow cytometry analysis of the apoptotic rate of podocytes in each group. *P < 0.05, n=4. ABCG1: ATP-binding cassette transporter G1.