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. 2020 May 30;10(16):7100–7110. doi: 10.7150/thno.45939

Figure 2.

Figure 2

NLRP3 inflammasome gene deletion reduces PCSK9 expression. (A) to (C) PCSK9 expression in MPMs at both intracellular and extracellular levels. Western blot quantification is shown as fold change vs. WT control (considered as baseline=1). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. control or indicated group. (D) PCSK9 secretion in serum. (E) and (F) PCSK9 expression in different tissues. Mice were given LPS and ATP by intraperitoneal route 6 h before collection of blood. Inhibition of PCSK9 secretion is much less in IL18-/- mice than in IL-1β-/- mice. ****P<0.0001 vs. WT in LPS + ATP group or indicated group. Scale bar: 20 µm. (G) PCSK9 secretion in serum with or without neuIL-1β pretreatment. Western blots in each group were performed with the same protein concentration and the same film exposure time. Data represent the mean ± SD of independent experiments (n=5 mice per genotype in cell experiments and n=7 mice per genotype in animal experiments), each performed in triplicate.