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. 2020 Jun 1;10(16):7193–7210. doi: 10.7150/thno.44877

Figure 4.

Figure 4

miR-1291 targets the ERRα pathway. (A) The Venn diagram displayed the overlaps between four different gene sets. (B) Levels of ESRRA and PGC-1A mRNAs in PANC-1 and MDA-MB-231 cells after transfection with miR-1291. The data are the mean ± SD (n = 6). (C) Western blot analysis of ERRα after transfection with miR-1291. Data are mean ± SD (n = 3). (D) MDA-MB-231 cell lines were stained for ERRα. (E) ERRα target gene mRNA levels measured under the same conditions described above. Data are mean ± SD (n = 6). (F) Bioinformatics analysis revealing two putative MRE sites for miR-1291 within the 3'UTR of ESRRA transcript. The seed sequence of miR-1291 is underlined. ESRRA 3'UTR luciferase reporter activities were determined. Data are mean ± SD (n = 5). (G) The sequence of ESRRA 3'UTR bearing the mutated MRE sites is shown. The ESRRA 3'UTR luciferase reporter activities were detected with two ERRα-Reporter-MUT plasmids. Data are mean ± SD (n = 5).