Fig. 3.

Effects of AMPA and ATP (or BzATP) alone or in combination on basal [³H]D-ASP release: a Effects of AMPA and ATP alone or in combination on basal [³H]D-ASP release and their antagonism by NBQX. After 38 min of perfusion with standard medium, synaptosomes were exposed for 4 min to standard medium containing AMPA (100 μM), ATP (1 mM), or both drugs together. When used, the antagonist NBQX (10 μM) was added to the medium 8 min before the agonists. All results are expressed as areas under the release/time curves (AUCs). Each bar represents the mean ± SEM of 3 different experiments, each run in triplicate. *P < 0.05 vs basal release. **P < 0.01 vs basal release. b Effects of BzATP and AMPA alone or in combination and their antagonism by A438079 and by NBQX. The effects of BzATP were tested at two concentrations (10 and 100 μM), each in the presence or absence of AMPA (100 μM). Exposure times to agonists and antagonists (A438079 and NBQX) are those described in panel A. Each bar represents the mean ± SEM of 3 different experiments, each run in triplicate. *P < 0.05 vs basal release. **P < 0.01 vs basal release