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. 2020 Jun 22;5(25):15476–15486. doi: 10.1021/acsomega.0c01618

Figure 3.

Figure 3

Alteration of autophagy in FLV- and FLV-SNED-treated cells: Relative expression of autophagy-related LC3B mRNA in MCF-7 cells at indicated time points after treatment with DMSO, SNED, FLV, or FLV-SNED (10 μM). *P < 0.05 FLV vs DMSO at 16 and 24 h, **P < 0.01 FLV-SNED vs SNED at 16 and 24 h. Two experiments performed in duplicate (A). MCF-7 cells were treated with FLV, FLV-SNED, or DMSO and were incubated with or without chloroquine (CQ, 25 μM). Lysates were immunoblotted with anti-LC3 antibody. Representative immunoblot of LC3 proteins, LC31 (18 kDa) and LC3II (16 kDa). Actin served as loading control (B). Densitometric quantification of LC3II. ****P < 0.0001 FLV + CQ vs DMSO + CQ, **P < 0.01 FLV vs FLV-SNED, *P < 0.05 FLV-SNED + CQ vs FLV + CQ. Four experiments performed in duplicate (C).