Skip to main content
. 2020 Jun 22;5(25):15476–15486. doi: 10.1021/acsomega.0c01618

Figure 5.

Figure 5

Lysosomal activity in FLV- and FLV-SNED-treated cells: Relative expression of the lysosomal marker, LAMP-1, at indicated time points following FLV or FLV-SNED treatment of MCF-7 cells, compared to DMSO and SNED *P < 0.05 FLV vs DMSO at 8 and 16 h, **P < 0.01 FLV vs DMSO at 24 h, **P < 0.01 FLV-SNED vs SNED at 8 and 16 h, ***P < 0.001 FLV-SNED vs SNED at 24 h. Two experiments performed in duplicate (A). MCF-7 cells were treated with FLV, FLV-SNED, or DMSO at the indicated time points. Lysates were immunoblotted with anti-LAMP-1 antibody. Representative immunoblot of glycosylated LAMP-1 (90–120 kDa). α-Tubulin served as loading control (B). Densitometric quantification of glycosylated LAMP-1 at different time points. ***P < 0.001 FLV-SNED vs DMSO at 24 h, +P < 0.05 FLV-SNED vs FLV at 24 h. Four experiments performed in duplicate (C). Representative fluorescent photomicrographs of LAMP-1; bar = 25 μm (D).