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. 2020 Jun 22;5(25):15476–15486. doi: 10.1021/acsomega.0c01618

Figure 6.

Figure 6

Effect of FLV and FLV-SNED on AMPK, MAPK, and mTOR signaling: MCF-7 cells were treated with FLV, FLV-SNED (10 μM), or DMSO for 24 h in the presence or absence of chloroquine (CQ, 25 μM; final, 6 h). Lysates were immunoblotted with antibodies to AMPK, pAMPK, ERK 42/44, pERK 42/44, and LC3. Representative immunoblots of corresponding proteins. Actin served as loading control (A). Densitometric quantification of pAMPK/AMPK. *P < 0.05 FLV + CQ vs DMSO + CQ, **P < 0.01 FLV-SNED vs FLV, ***P < 0.001 FLV-SNED + CQ vs DMSO + CQ, +P < 0.05 FLV-SNED + CQ vs FLV + CQ. Three experiments performed in duplicate (B). The same lysates were immunoblotted with antibodies to S6 kinase, phospho-S6 kinase, and LC3. Representative immunoblots of corresponding proteins. Actin served as loading control. Phosphorylated S6 kinase, 85 kDa isoform (pp85), is reduced in FLV-SNED (C).