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. 2020 Jul 2;53:27. doi: 10.1186/s40659-020-00295-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — circ_ANRIL aggravates OGD/R-induced cell damage. a Upregulation of circ_ANRIL was induced by transfection with circ_ANRIL overexpressing plasmid. The expression of circ_ANRIL in OGD/R-treated HBMECs after plasmid transfection was detected by qRT-PCR. b The results of CCK-8 assay showed that overexpression of circ_ANRIL significantly aggravated the reduction of proliferation viability induced by OGD/R treatment in HBMECs. c The results of LDH assay showed that circ_ANRIL overexpression aggravated OGD/R-induced cell cytotoxicity. d The apoptosis rate of OGD/R-induced HBMECs was analyzed by flow cytometry. e The expression of Bcl-2 and Bax was analyzed by western blotting. The promotion effect of circ_ANRIL on OGD/R-induced apoptosis was verified by the changes in protein expression of Bcl-2 and Bax. **p < 0.01, ***p < 0.001. n = 3