Table 2.
Evaluation of the raw yield and quality of TruDrop libraries when sequenced on the NovaSeq
| Library | Sequencer | i7 | i5 | Targeted inDrop Read Depth | Observed inDrop Reads | Average % of the lane | Percent perfect index reads | Mean transcript Quality Score | Mean Barcodes and UMI quality score |
|---|---|---|---|---|---|---|---|---|---|
| TruDrop Mouse 4 | NovaSeq 6000 | CCGCGGTT | AGCGCTAG | 50,000,000 | 53,655,662 | 0.64% | 96.99% | 35.57 | 36.22 |
| TruDrop Mouse 5 | NovaSeq 6000 | TTATAACC | GATATCGA | 50,000,000 | 44,554,464 | 0.53% | 94.13% | 35.53 | 36.19 |
| V2 Mouse 2 + 15% illumina PhiX | NextSeq | GATATCGA | – | 65,000,000 | 57,847,546 | 37.68% | 91.72% | 33.06 | 33.02 |
| V2 Mouse 3 + 15% illumina PhiX | NextSeq | GCCAAT | – | 65,000,000 | 64,673,114 | 42.14% | 92.64% | 33.12 | 33.14 |
| V2 Mouse 4 + 99% Illumina PhiX | NovaSeq 6000 | CTTGTA | – | 50,000,000 | 10,985,817 | 0.09% | – | – | – |
| V2 Mouse 5 + 99% Illumina PhiX | NovaSeq 6000 | GTGAAA | – | 50,000,000 | 10,144,573 | 0.08% | – | – | – |
| V2 Mouse 4 + 0% Illumina PhiX | NextSeq | CTTGTA | – | 100,000,000 | 97,872,275 | 24% | – | 31.89 | 28.67 |
| V2 Mouse 5 + 0% Illumina PhiX | NextSeq | GTGAAA | – | 100,000,000 | 92,742,820 | 23% | – | 32.03 | 28.09 |