Fig. 3.

dose response curves Cells were seeded into a 96 well plate at a density of 1.5 (HT-29, MDA-MB) and 0.5 × 10⁴/well (MCF-7), incubated 24 h to a confluence of 50%, then cells were treated with increasing concentrations of the 14 selected drugs for 48 h. Viability was assessed using the MTT-Assay and curves were obtained using the four-parameter variable slope function of Graphpad Prism. Exemplarily the resulting curves for Nutlin-3 and DCA are shown for the three cell lines