Fig. 1. Overview of Human1 generation and curation.

A simplified illustration of the key steps involved in the generation of Human1 from HMR2, Recon3D, and iHsa. The bottom of the diagram represents the ongoing open-source curation of Human1 using input from databases, literature, other models, and the scientific community. The four side panels provide further detail into selected Human1 features: extensive reaction mass and charge balancing to achieve 100% stoichiometric consistency; incorporation of new enzyme complex information; mapping model components to standard database identifiers; version-controlled and open-source model curation framework. In the bar graphs in the upper left panel, “Balanced” reactions represent the number of mass-balanced reactions, “Consistent” metabolites are the number of stoichiometrically consistent metabolites, and “R3D model” is the model version of Recon3D.