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RNA-seq analysis of the PGCLC induction process. (A) Unsupervised hierarchical clustering (UHC) of gene expression in hiPSCs, iMeLCs, PGCLCs, and gonadal hPGCs. RNA-seq was performed on two biological replicates (1 and 2) for each cell type. (B) PCA of RNA-seq data. (C) Heat map of RNA-seq data. (D) GO analysis of the genes during hPGCLC specification through iMeLCs.
