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. 2020 Jul 2;10:10866. doi: 10.1038/s41598-020-66483-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dual luciferase transient expression assay to probe interaction of transcription factor MdPIF4 and MdYUCCA8a promoter in tobacco leaves. (a) Vector construction diagram. (b) The activity of firefly and renilla luciferase in tobacco leaves was measured 3 d after infiltration. Error bars show SEs of three independent experiments with at least four replicate reactions. The asterisks indicate statistical significance (p < 0.05).