Table 4.

MIC of various antimicrobial agents in E. coli cells transformed with deduced RND-type efflux pump gene from K. pneumoniae.

Antimicrobial agent	Minimum inhibitory concentration (μg/ml)
	Host: E. coli KAM33										Host: E. coli KAM32
	pSTV28	pKAC28M	pKAB28	pKEF28	pKGH28	pKJK28	pKLM28	pKRS28	pKTU28	pKVWX28	pSTV28	pKC28	pKD28
	vector	acrAB	oqxAB	kexEF	eefAB	kexJK	cusAB	kexRS	kexTU	kexVWX	vector	kexC	kexD
Oxacillin	8	1,024	> 1,024	256	256	8	8	8	8	32	16	512	n.t.
Cloxacillin	4	512	64	64	128	4	4	4	4	128	4	32	4
Norfloxacin	0.03	0.25	0.5	0.03	0.125	0.03	0.03	0.03	0.03	0.03	0.03	0.03	0.03
Erythromycin	4	512	4	64	64	4	4	4	4	4	4	4	128
Kanamycin	1	1	1	1	1	1	1	1	1	1	1	1	1
Tetracycline	0.5	4	0.5	2	4	0.5	0.5	0.5	0.5	0.5	0.5	0.5	2
Novobiocin	1	512	8	64	32	1	2	2	1	8	2	8	8
Acriflavine	8	128	64	16	128	8	8	8	8	16	2	2	8
Benzalkonium Cl	4	32	32	8	16	4	4	4	4	4	4	4	8
Hoechst33342	0.5	8	4	4	> 16	0.5	0.5	0.5	0.5	0.5	0.5	0.5	1
Ethidium Br	4	1,024	128	128	1,024	4	4	4	4	4	4	4	32
Rhodamine 6G	8	> 1,024	512	256	1,024	8	8	8	8	8	8	8	32
SDS	64	> 1,024	> 1,024	> 1,024	> 1,024	512	64	64	64	64	64	> 1,024	256
TPPCl	8	1,024	512	256	1,024	8	8	8	8	8	8	8	256
Cholate	5,000	> 40,000	5,000	20,000	10,000	5,000	10,000	5,000	5,000	20,000	10,000	40,000	10,000

n.t. not tested.