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. 2020 Jun 25;13:85. doi: 10.3389/fnmol.2020.00085

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Copyright © 2020 O’Hare Doig, Santhakumar, Fehily, Raja, Solomon, Bartlett, Fitzgerald and Hodgetts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagram demonstrating regions of interest (ROIs) in a selected section of the spinal cord for immunohistochemistry. Immunohistochemical labeling was visualized in a selected section of the spinal cord, in the immediate penumbra surrounding the lesion site (cyst). Images were collected from six 200 μm × 200 μm regions of interest (black box; dorsorostral, centrorostral, ventrorostral, dorsocaudal, centrocaudal, and ventrocaudal) immediately adjacent to the injury site (A; not to scale). Stitched ×10 gray scale fluorescent image demonstrates cord injury site and evidence of cystic structures (B).