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. 2020 Jul 2;10:10921. doi: 10.1038/s41598-020-67872-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of iPSCs from TOF patients and healthy relatives and their differentiation to cardiomyocytes. (A) Families with TOF patients (indicated by a red heart) and healthy family members. For each generated cell line, the individual clone identifier is provided (n = 3 for corresponding individual). Moreover, it is indicated whether genomic DNA was taken from blood for whole genome sequencing and/or from RV for targeted re-sequencing. (B) Generation of iPSCs and their differentiation to CMs. The gene expression of the pluripotency markers was also investigated at mRNA level by RT-PCR (here for iPSCs of TOF-01 exemplarily). The grouping of blots was cropped from different parts of the same gel, or from different gels. Uncropped blots are available in Supplementary Fig. S6. (C) Morphology of the generated iPSC colonies. Magnification 10×. Scale bar 100 µm. (D) Differentiation efficiency of CMs (each n = 3) at d15 and d60 between patients (light grey) and healthy relatives (dark grey). Cardiac differentiation efficiency is based on the percent of cTnT-positive CMs. CMs: cardiomyocytes; RV: right ventricle; TOF: Tetralogy of Fallot.