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. 2020 Jul 2;10:10950. doi: 10.1038/s41598-020-67936-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in skin conductance during the heat stimulus period. (a) Time course of NFSC during heat stimulation. The white, yellow, orange and red circles indicate NFSC values during control (32 °C), 46 °C, 47 °C and 48 °C heat stimulation. (b) Comparison of the area under the curve (AUC) of NFSC during heat stimulation with various temperatures indicated no significant differences between them. (c) Time course of nSCL during heat stimulation. nSCL reflected heat intensity well. (d) AUC of nSCL between the different temperatures used for heat stimulation. There were significant differences among all pairs of heat stimuli, including control (32 °C) stimulation. One-way ANOVA post hoc Student–Newman–Keuls test, P < 0.05, * 32 °C vs. all other stimuli, † 46 °C vs. all other stimuli, ‡ 47 °C vs. all other stimuli, § 48 °C vs. all other stimuli.