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. 2020 Jul 2;10:10857. doi: 10.1038/s41598-020-67675-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conditional disruption of PHRF1 gene in mice. (a) Schematic representation of the mouse PHRF1 gene flanked by two loxP sites between exon 1 and exon 10 of PHRF1 gene. (b) Immunoblot analysis of hippocampal extracts prepared from PHRF1^fl/fl and PHRF1^Δ/Δ littermates. α-Tubulin was used as a loading control. All Western blots were processed in identical conditions and cropped from Supplementary Fig. S4a. (c) Comparison of body weight from PHRF1^fl/fl and PHRF1^Δ/Δ littermates (n = 5).