Figure 3.

Learning and memory test in PHRF1^Δ/Δ mice. (a) Novel object recognition. In the test phase, the discrimination ratio of the familiar object and novel object were quantified (n = 5, all males). (b) Y-maze test. Each mouse was placed at the end of one arm and allowed to move freely through the maze during an 8-min session; the number of alternations were analyzed. Data are mean ± SEM. *p < 0.05, two-tailed unpaired Student t-test. (PHRF1^fl/fl n = 13, 11 males, 2 females; PHRF1^Δ/Δ n = 12, 9 males, 3 females) (c) An individual mouse in the PHRF1^fl/fl and PHRF1^Δ/Δ groups was placed in a water maze and allowed to swim to the hidden platform four times a day for five days. The swimming path and duration in quadrants of the water pool were recorded, and the latency to locate the platform was averaged and analyzed each day. *p < 0.05 by student’s t-test. (n = 12; 8 males, 4 females). (d) For the probe trial on Day 6, the hidden platform was removed and the number of times the mouse swam to the empty area in 90 s was recorded and analyzed (right panel). * p < 0.05 by student’s t-test. (n = 12; 8 males, 4 females).