Figure 2:

COMSOL simulation of mass transfer in “flow-free” cell culture channels. (A) Diffusion of a model biomolecule from the outer flow channel into the center culture channels. (Inset image) Visual representation of the COMSOL simulations showing diffusion across the device. (B) Simulation of biomolecules between the two flow-free culture channels. (Inset image) Visual representation of the COMSOL simulation. In both (A) and (B) biomolecules were approximated at 140 nM SDF-1α in the source channels(C) Simulation of the oxygen diffusion from outside of the device, through PDMS to cell culture channel. External oxygen was approximated to be ≈21%.