Figure 3:

Simultaneous co-culture of MDA-MB-231 cells and ASCs alters breast cancer cell growth and morphology. (A) The growth of the MDA-MB-231 cells was observed in single and co-culture using an initial cell density of 0.5×105 cells/mL. (B) Morphological changes in MDA-MB-231 cells in single culture and co-culture was determined by calculating the aspect ratio: measuring the ratio of the major cell axis length to minor cell axis length of individual cells. A minimum of 500 MDA-MB-231 cells was analyzed for each time point. Single culture and co-culture data were compared by student’s t-test with a statistically significant value set at p<0.05.