Figure 5:

MDA-MB-231 cells orient themselves towards the ASC population during simultaneous co-culture. (A) The angle was calculated with the cell major axis line with respect to the horizontal line. The angles were taken within 0° to 90° where 0° refer no directionality of cells in the parallel position of the channel, and 90° indicates 100% directionality of cells in the perpendicular position of channels. All angles were measured (B) when MDA-MB-231 cells were cultured in single culture. (C) when MDA-MB-231 cells were cultured in Co-culture with ASCs for 72 h. The warm color represents angle in the range of 45° to 90°. A minimum 500 cells were analyzed for angle measurement.