Figure 6:

Co-cultured cancer cells show drug resistance to 20 nM Paclitaxel. The viability of MDA-MB-231 cells treated with either a DMSO control or Paclitaxel was measured on-chip after 3 days of Paclitaxel treatment in single cultured and co-cultured experiments. MDA-MB-231 cells were incubated with Calcein AM (2.5 μM) and Ethidium homodimer-1 (4 μM) after terminating the experiment. Viability experiments were conducted triplicate and the statistical differences between two groups were determined by the student’s t-test with a statistically confidence interval value set at p<0.05.