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. 2020 Jul 2;10:10859. doi: 10.1038/s41598-020-67633-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mirror symmetry algorithm for automatic orientation and cropping. (a) A single slice from a CT reconstruction, pre-cropping. In (b), slices of the CT have been projected in the cranial/caudal direction to create a single image and the image thresholded to show the bones, revealing the symmetry of the ribcage. The red line indicates the line-of-best-symmetry as found by the algorithm, passing through the spine and sternum, allowing us to register their positions. (c) Using this result, the algorithm automatically corrects the orientation of the sample, and crops the image to the bones that act as a boundary for lung tissue; (d) shows a rotated and cropped slice.