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. 2020 Jul 2;10:10859. doi: 10.1038/s41598-020-67633-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A diagram of the image acquisition process. (a) Experimental schematic: The mouse is mounted on a rotation stage in front of the x-ray beam, generated by an electron beam hitting the liquid–metal jet anode, pumped under high pressure to retain a laminar flow. A small-animal ventilator ensures that the detector acquires images at a rate that is synchronized to the breathing pattern of the mouse. The vacuum tube (not shown) lies between the mouse and the detector; (b) An example of a single projection image collected by the detector as the animal is rotated in front of it; (c) A single slice from the CT reconstruction of the projections from a single time point (Adapted from²⁵).