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. 2020 Jul 2;10:10890. doi: 10.1038/s41598-020-67508-2

Table 2.

Comparison of total phenolics contents, flavonoids contents, and antioxidant activities of OJ among extraction solvents. Means in a row followed by same superscript letters are not significantly different according to Duncan’s test at p < 0.05.

Extraction solvent, temperature, and time
Subcritical water 220 °C and 15 min Methanol 25 °C and 2 h Ethanol 25 °C and 2 h Methanol 60 °C and 2 h Ethanol 60 °C and 2 h
Total phenolics content (mg GAE/g) 39.9 ± 4.1c 6.87 ± 0.4ab 3.5 ± 0.3a 10.7 ± 1.3b 8.0 ± 2.6b
Total flavonoids content (mg QE/g) 11.4 ± 0.6c 4.03 ± 0.2a 3.6 ± 0.8a 5.8 ± 1.2b 6.1 ± 1.0b
DPPH-radical-scavenging ability (%) 90.3 ± 2.2c 89.57 ± 1.7bc 70.9 ± 4.3a 89.7 ± 0.3bc 81.0 ± 9.2b
ABTS-radical-scavenging ability (%) 96.0 ± 2.9d 57.70 ± 3.0b 19.6 ± 0.8a 78.7 ± 9.9c 57.0 ± 11.3b
FRAP antioxidant activity (mg TE/g) 662.4 ± 17.2c 328.9 ± 11.0b 130.6 ± 18.0a 318.7 ± 6.1b 177.1 ± 7.4a

The data are mean ± SD values (n = 3).