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. 2020 Jul 2;10:10913. doi: 10.1038/s41598-020-67988-2

Figure 6.

Figure 6

Cleaved VAMP2 does not act as a dominant negative for DCV fusion. (A) Schematic representation of the cleaved VAMP2 construct (cl-VAMP2) with the transmembrane domain remaining (blue). (B) Histogram of DCV fusion events in control (black) and cl-VAMP2 infected neurons (infected at DIV 8 with NPY-pHluorin and a control or cl-VAMP2 construct and imaged at DIV 14–16, orange). (C) Cumulative plot of DCV fusion events in control construct and cl-VAMP2 infected neurons. Shaded area represents SEM. Blue bars indicate 16 trains of 50 AP at 50 Hz interspaced by 0.5 s. (D) Cumulative DCV fusion events in control and cl-VAMP2 infected neurons during the first 3 bursts of stimulation. Blue bars indicate trains of 50 AP at 50 Hz interspaced by 0.5 s. (E) Average DCV fusion events per cell for control (n = 13, N = 3) and cl-VAMP2 (n = 20, N = 3) infected neurons. Mann–Whitney U test: p = 0.29 non-significant (ns). Bars represent mean + SEM. Detailed statistics are shown in Supplementary Table S1.