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. 2020 Jul 2;10:10835. doi: 10.1038/s41598-020-67783-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-of-additional of VDR agonists. Mock or DENV infected HEK293T/17 cells were pre- and post-treated with 0.01% DMSO and 10 µM of ZD-1, ZD-2, ZD-3, ZD-5 and ZD-6 at various time points as shown in the diagram. (A) At 24 h.p.i. the treated cells were collected and the levels of infection determined using flow cytometry. (B) In parallel, the supernatant of the treated cells were evaluated for virus titer by standard plaque assay. p value; *< 0.05, **< 0.01, ***< 0.001 for significance. The experiment was performed independently in triplicate with duplicate plaque assay.