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. 2020 Jul 2;10:10835. doi: 10.1038/s41598-020-67783-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VDR agonist activity on viral protein and viral genome copy number. (A–E) HEK293T/17 cells were mock or DENV 2 infected followed by treatment with 0.01% DMSO or 10 µM of VDR agonists (ZD-1, ZD-2, ZD-3, ZD-5 and ZD-6) at the indicated time points. Expression of DENV structural protein (E) and non-structural proteins (NS1, NS3 and NS5) was determined by western blot assay, using GAPDH as an internal control. White spaces separate different antibody probing. (F) In parallel, the supernatant were collected the level of the viral genome copy number determined by qRT-PCR. Viral genome copy number was calculated by comparing with tenfold serial dilution of a DENV NS1 standard control. p value; *< 0.05, **< 0.01, ***< 0.001 for significance. All experiments were performed independently in triplicate.